Villalva, D. G. has pursued PhD in Material Science - Nano science from Sapienza University of Rome, Italia. Now, she is a researcher at UNICAMP (Institute of Chemistry), Brazil.
Cubosomes are lyotropic liquid crystals nanoparticles that have been an assertive option to carry drugs and biomolecules. They can offer high efficiency of entrapment, specificity to targets and responsivity to external stimuli. Hyaluronic acid (HA) is a natural biopolymer, containing functional groups for chemical modifications that favor the production of biodegradable, stable, thermo-sensitive and injectable hydrogels. Herein, anionic and cationic cubosomes, composed of phytantriol, Nile Red dye and stabilized by Pluronic F127 were immobilized in partially oxidized HA crosslinked with adipic acid dihydrazide (ADH). The systems were assessed for their ability of dispersion and distribution by optical fluorescence confocal microscopy images and evaluated by the capacity of sodium-diclofenac controlled delivery. These systems may allow for a depot in specific regions of the body, helping drugs, peptides or proteins act more locally and with the more prolonged release if compared with pure gels. Cubosomes were prepared by tip ultrasonication method and characterized by DLS and SAXS, while hydrogels were prepared oxidizing HA with NaIO4 and crosslinking with ADH in the presence of cubosomes. Our results show a homogeneous distribution of cubosomes into the HA/ADH hydrogel and a synergic effect of hydrogel and cubosomes in slowing down the release of sodium diclofenac, being more effective to cationic nanoparticles. These injectable hydrogels loaded with cubosomes display potential as modulable carriers of bioactive compounds in the biomedical field.